The genomic analysis of both strains revealed a diversity of carbohydrate-active enzymes.
Furthermore, strain F7 was able to degrade pectin, mannan, and lichenan. After cultivation on steam-pretreated sugarcane bagasse-based medium, the supernatant of the strains F1 and F7 exhibited enzymatic activity against different substrates, such as arabinan, rye arabinoxylan, β-glucan, starch, CMC, xylan, and chitin. Among the 80 bacterial isolates that were screened for enzymatic activity, two Streptomyces strains (named as F1 and F7) exhiting higher endoglucanase and endoxylanase activities were selected for biochemical and genomic characterization.
Subsequently, genomes of Streptomyces strains were sequenced, annotated, and interpreted to correlate their genetic contents with biochemical properties. Streptomyces strains isolated from soil were functional screened based on their cellulolytic and hemicellulolytic capacities by enzymatic plate assays containing carboxymethylcellulose (CMC) and beechwood xylan as sole carbon source. Here, we employed functional screening along with genomic sequencing for identification of novel lignocellulolytic Streptomyces strains. are notorious plant biomass decomposers in soil environments, but only few strains were biochemically and genetically characterized.
